koko体育app

欢迎来到《koko体育app 学报（医学版）》
葛贤明, 刘亚明, 张配, 等. 2-脱氧-D-葡萄糖联合羟基喜树碱协同诱导乳腺癌细胞凋亡的作用[J]. koko体育app 学报（医学版）, 2019, 50(4): 527-532.
引用本文: 葛于诚, 刘亚明, 张配, 等. 2-脱氧-D-冬枣糖联办羟基喜树碱携手成脂乳线癌肿瘤细胞凋亡的能力[J]. 重庆师范大学学报（临床版）, 2019, 50(4): 527-532.
GE Xian-ming, LIU Ya-ming, ZHANG Pei, et al. Synergistic Effect of 2-deoxy-D-glucose Combined with Hydroxycamptothecin on Apoptosis of Breast Cancer Cells[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(4): 527-532.
Citation: GE Xian-ming, LIU Ya-ming, ZHANG Pei, et al. Synergistic Effect of 2-deoxy-D-glucose Combined with Hydroxycamptothecin on Apoptosis of Breast Cancer Cells[J]. Journal of Sichuan University (Medical Sciences), 2019, 50(4): 527-532.

2-脱氧-D-葡萄糖联合羟基喜树碱协同诱导乳腺癌细胞凋亡的作用

Synergistic Effect of 2-deoxy-D-glucose Combined with Hydroxycamptothecin on Apoptosis of Breast Cancer Cells

  • 摘要:
      目的  探究2-脱氧-D-葡萄糖(2-deoxy-D-glucose, 2-DG)联合羟基喜树碱(hydroxycamptothecin, HCPT)对乳腺癌细胞抗肿瘤活性的影响及其机制。
      方法  采用2-DG(0、1.25、2.5、5、10、20 mmol/L)、HCPT(0、5、10、20、40 μmol/L)单独作用以及5 mmol/L 2-DG与各浓度HCPT联合作用于乳腺癌细胞MDA-MB-231与MCF-7，使用MTT法检测细胞增殖；溴化丙啶(propidium iodide，PI)检测5 mmol/L 2-DG、10 μmol/L HCPT单独作用以及联合作用对MDA-MB-231细胞的凋亡作用；不同浓度2-DG作用于MDA-MB-231细胞6 h后，测定细胞内ATP含量变化；Western blot检测MDA-MB-231细胞Akt、P-Akt、Bcl-2/Bax、PARP、Caspase-8和Caspase-3蛋白的表达。
      结果  5 mmol/L 2-DG与HCPT联合具有协同作用，处理48 h结合指数(CI < 1)，合用组凋亡率均高于单用组(P < 0.05), 同时两者合用抑制Akt蛋白的磷酸化以及增加了Caspase-3蛋白的活化，增加了对PARP蛋白的剪切。
      结论  2-DG联合HCPT可以协同诱导乳腺癌细胞凋亡，其机制可能是抑制肿瘤细胞能量生成以及抑制Akt蛋白的磷酸化和增强Caspase-3蛋白的活性。
     
    Abstract:
      Objective  To investigate the effect of 2-deoxy-d-glucose (2-DG) combined with hydroxycamptothecin (HCPT) on anti-tumor activity of breast cancer cells and its mechanism.
      Methods  MDA-MB-231 and MCF-7 breast cancer cells were incubated with varying concentrations of 2-DG (0, 1.25, 2.5, 5, 10, 20 mmol/L), HCPT(0, 5, 10, 20, 40 μmol/L) and 2-DG (5 mmol/L) combined with HCPT. Cell viability was measured using the MTT assay; Propidium iodide (PI) detected the apoptosis of MDA-MB-231 cells by 5 mmol/L 2-DG, 10 μmol/L HCPT alone or in combination; MDA-MB-231 cells were treated with 2-DG (0, 2.5, 5, 10, 20 mmol/L) and the level of ATP was detected by ATP kit; the expression of Akt, p-Akt, Bcl-2/Bax, PARP, Caspase-8 and Caspase-3 proteins in MDA-MB-231 cells were measured by Western blot assay.
      Results  The combination of 2-DG (5 mmol/L) and HCPT had a synergistic effect. The 48 h combination index (CI < 1) was higher than that of the single-use group (P < 0.05). At the same time, the combination of the two drugs inhibits the phosphorylation of Akt protein and increases the activation of Caspase-3 protein, thereby increasing the cleavage of PARP proteins.
      Conclusion  The combination of 2-DG and HCPT can synergistically induce the apoptosis of breast cancer cells, which may be caused by inhibiting the energy generation of tumor cells, inhibiting the phosphorylation of Akt protein and enhancing the activity of caspase-3 protein.
     
    • HTML全文
© 2019 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取🅠 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!