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夏卫维, 郑翔, 潘倩等. 18月龄与3月龄SD大鼠脑干Caspase-3阳性凋亡细胞的分布比较[J]. koko体育app 学报（医学版）, 2014, 45(6): 928-932.
引用本文: 夏卫维, 郑翔, 潘倩等. 11月龄与1月龄SD大鼠脑干Caspase-3弱阳凋亡血细胞的分布图制作更[J]. 北京综合大学学报（医学专业版）, 2014, 45(6): 928-932.
XIA Wei-wei, ZHENG Xiang, PAN Qian. et al. The Distribution of Caspase-3 Positive Apoptotic Cells in 18 Months Old SD Rat’s Brainstem Compared with That in 3 Months Old Rat[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(6): 928-932.
Citation: XIA Wei-wei, ZHENG Xiang, PAN Qian. et al. The Distribution of Caspase-3 Positive Apoptotic Cells in 18 Months Old SD Rat’s Brainstem Compared with That in 3 Months Old Rat[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(6): 928-932.

18月龄与3月龄SD大鼠脑干Caspase-3阳性凋亡细胞的分布比较

The Distribution of Caspase-3 Positive Apoptotic Cells in 18 Months Old SD Rat’s Brainstem Compared with That in 3 Months Old Rat

  • 摘要: 目的 观察和比较老龄（18月龄）和年轻（3月龄）SD大鼠脑干Caspase-3阳性凋亡细胞的分布，研究脑干神经元在自然老化的过程中出现凋亡的分布规律。方法 将健康雄性SD大鼠分为18月龄与3月龄2组，每组3只，处死后取脑干部分行石蜡包埋和连续冠状面切片，片厚6 μm，每隔33张取一片，共取3套（每套30张）切片，分别行常规HE染色、免疫组织化学检测与TUNEL检测。并将获得的Caspase-3阳性结果标记到图谱上后进行三维合成。结果 与3月龄大鼠相比，18月龄大鼠脑干相关区域的Caspase-3阳性细胞数量更多、分布范围更广，尤其在孤束核和脑桥网状核增加明显。结论 随着大鼠年龄的增长，脑干发生细胞凋亡的区域增大、数量增多。  
    Abstract: Objective To study whether there is apoptosis in brainstem neurons while aging by oberving the distribution of Caspase-3 positive apoptotic cells in in brainstem of young and old SD rats. Methods Healthy male SD rats were divided into 2 groups (3 and 18 month-old respectively), 3 rats each group. Brainstem specimens were treated followed the brainstem’s common paraffin embedding, sectioning and HE staining procedures (sections were 6 μm in thickness). The sections were also determined by Caspase-3 immunostaining and TUNEL. The Caspase-3 positive cells on the rat stereotaxic atlas were drew, then composed the sections into a 3D model. Results Compared to 3 month-old rats, there were more Caspase-3 positive neurons in the brainstem and the positive neurons were distributed more extensively in 18 month-old rats spectially in nucleus of solitary tract and pontine reticular nuclei. Conclusion More neurons suffer apoptotic changes in the brainstem while aging.  
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