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庞富文, 蔡华伟, 李玉豪等. 放射性131I标记胶原-壳聚糖复合微球的制备及其杀伤肝癌细胞的体内研究[J]. koko体育app 学报（医学版）, 2018, 49(1): 24-28.
引用本文: 庞富文, 蔡华伟, 李玉豪等. 有害性131I标上胶原-壳聚糖pp微球的分离纯化还有破坏肝癌細胞的里面探究[J]. 河南大家学报（医美版）, 2018, 49(1): 24-28.
PANG Fu-wen, CAI Hua-wei, LI Yu-hao. et al. Preparation of 131I Labeled Collagen-Chitosan Microspheres and It’s Antitumor Effect on Human Liver Cancer[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(1): 24-28.
Citation: PANG Fu-wen, CAI Hua-wei, LI Yu-hao. et al. Preparation of 131I Labeled Collagen-Chitosan Microspheres and It’s Antitumor Effect on Human Liver Cancer[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(1): 24-28.

放射性131I标记胶原-壳聚糖复合微球的制备及其杀伤肝癌细胞的体内研究

Preparation of 131I Labeled Collagen-Chitosan Microspheres and It’s Antitumor Effect on Human Liver Cancer

  • 摘要: 目的 用壳聚糖及胶原这两种可降解生物材料，制备能用于131I标记的放射性核素微球并探究其对肝癌的治疗效果。方法 以Ⅰ型胶原蛋白和壳聚糖为主要材料，戊二醛为交联剂，利用乳化交联法制备胶原-壳聚糖复合微球。电镜下观察微球表征，测量并统计微球的粒径。通过氯胺T法对微球进行131I标记，计算标记率。等量131I-微球分别置于PBS和人血清中192 h，探究其体外稳定性。BALB/C裸鼠皮下一次性注射人肝癌细胞HepG2约106个，构建裸鼠人肝癌细胞模型。28 d后，131I-微球、空白微球、PBS分别一次性注入荷人肝癌裸鼠模型的皮下瘤中（每组5只），治疗7 d后处死裸鼠。取血和心、肺、肝、脾、胃、小肠、肾、肌肉、脑、骨、甲状腺、皮下肝癌组织检测标记微球在裸鼠体内的分布，同时对皮下肝癌组织进行组织病理学HE染色，检测标记微球对肝癌细胞的杀伤效果。结果 本研究成功制得胶原-壳聚糖微球，电镜显示微球成球性好，表面光滑，粒径较均匀，平均粒径为(5.1±1.2) μm。其131I标记率为86.10%，置于PBS及血清192 h后，碘标记率分别为92.00%、83.00%，131I-微球依然保持高稳定性。治疗7 d后裸鼠体内分布实验显示放射性主要集中在皮下肿瘤组织，其他组织几乎没有放射性计数。皮下肿瘤组织病理切片显示PBS组见大量肝癌细胞，标记微球后周围的肝癌细胞大量死亡，空白微球组中微球周围仅有少量的炎性细胞，未引起局部强烈的免疫反应。后两组微球均未见明显降解。结论 本研究制得的胶原-壳聚糖具有很高的131I标记率、体内外稳定性及良好的肿瘤杀伤效果。  
    Abstract: Objective To prepare iodine-131(131I) labeled biodegradable microspheres with chitosan and collagen for treating liver cancer. Methods Collagen-chitosan microspheres (CCMSs) were prepared with type-Ⅰ collagen and chitosan using emulsification-chemical cross-linking method. The size of the CCMSs were determined by electron microscope. 131I-CCMSs were achieved using Chloramine-T. The labelling rate of 131I was recorded. The stability of 131I-CCMSs in vitro were evaluated in PBS or human blood serum through 192 h incubation. The HepG2 model was established in nude mice 28 d after subcutaneous injection of 106 HepG2 cells. The model mice were sacrificed 7 d after injection of 131I-CCMSs, blank microspheres, or PBS (five mice in each group) into the HepG2 tumor xenografts. Samples of various organs were collected to determine the distribution of 131I-CCMSs. The curative effect of 131I-CCMSs on liver cancer was assessed by staining with HE for histological analyses. Results CCMSs were synthesized with a smooth and spherical shape and an average diameter of (5.1±1.2) μm. A radiolabeling rate of 86.10% was achieved. 131I radio-loading remained stable: 92.00% in saline and 83.00% in human serum after 192 h incubation. 131I was mainly concentrated in the subcutaneous tumor tissues. Potent curative effects of 131I-CCMSs on subcutaneous tumor tissues were demonstrated. Conclusion Biodegradable CCMSs were successfully prepared and radiolabeled. The 131I-CCMSs exhibited potential curative effects on liver cancer, with high stability in vitro and in vivo.  
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