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古君, 蒙炜, 干昌平, 等. 主动脉中膜诱发全身炎症反应动物模型的建立[J]. koko体育app 学报（医学版）, 2018, 49(4): 604-607.
引用本文: 古君, 蒙炜, 干昌平, 等. 分手后脉中膜引发一身宫颈炎症化学反应甲壳动物建模方法的加入[J]. 北京高中学报（药学版）, 2018, 49(4): 604-607.
GU Jun, MENG Wei, GAN Chang-ping, et al. The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(4): 604-607.
Citation: GU Jun, MENG Wei, GAN Chang-ping, et al. The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(4): 604-607.

主动脉中膜诱发全身炎症反应动物模型的建立

The Establishment of Tunica Media-induced Systemic Inflammatory Responses Animal Model

  • 摘要: 目的 建立主动脉中膜诱导全身炎症反应的动物模型，证实主动脉夹层的特征性病理改变——主动脉中膜暴露于血液可以引起全身炎症反应。 方法 通过外科技术剥离供体C57/BL6小鼠主动脉中膜并进行匀浆，离心后取上清液注射入受体小鼠体内，利用ELISA技术检测不同时间点实验动物血清中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）变化规律。 结果 除基线水平外，小鼠体内注入主动脉中膜提取物后不同时间点血清中TNF-α、IL-6均升高（P<0.05），TNF-α和IL-6的峰值均出现在3 h和24 h。 结论 主动脉中膜与血液接触可以诱导实验动物全身炎症反应。  
    Abstract: Objective To establish animal model of tunica media-induced systemic inflammatory responses and verify the stability of established model. Methods Tunica media of aorta was captured from C57/BL6 mouse donor, followed by the intervention of homogenization. After centrifugation, the supernatant was harvested and injected into the acceptor of C57/BL6 mouse to mimic the featured pathological changes induced by the exposure of tunica media with circulation. ELISA assay was used to detect the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Results The levels of TNF-α, IL-6 in the model group were significant higher than those in the control group (P<0.05). There were two peak values of both TNF-α and IL-6 identified at 3 h and 24 h after the injection of supernatant in the model group. Conclusion The exposure of tunica media to circulation may induce systemic inflammatory responses.  
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