koko体育app

欢迎来到《koko体育app 学报（医学版）》
郭梅梅, 王永, 韩方海. siRNA干扰胃癌细胞内源性VEGF-C的基因和蛋白表达的实验研究[J]. koko体育app 学报（医学版）, 2013, 44(5): 740-743.
引用本文: 郭梅梅, 王永, 韩方海. siRNA影响食道癌细胞核内源性VEGF-C的染色体和血清表达方式的实践科学研究[J]. 四川省学校学报（医美版）, 2013, 44(5): 740-743.
GUO Mei-mei, WANG Yong, HAN Fang-hai. The Study of Interfering of Endogenous VEGF-C Genes and the Protein Expression Gastric Cancer Cell with siRNA Technique[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(5): 740-743.
Citation: GUO Mei-mei, WANG Yong, HAN Fang-hai. The Study of Interfering of Endogenous VEGF-C Genes and the Protein Expression Gastric Cancer Cell with siRNA Technique[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(5): 740-743.

siRNA干扰胃癌细胞内源性VEGF-C的基因和蛋白表达的实验研究

The Study of Interfering of Endogenous VEGF-C Genes and the Protein Expression Gastric Cancer Cell with siRNA Technique

  • 摘要: 目的 探索siRNA干扰技术阻断胃癌细胞内源性血管内皮生长因子-C (VEGF-C)的基因和蛋白表达的有效性。 方法 培养胃癌细胞株SGC7901,选择GenBank的Accession No.BC035212.1设计5条VEGF-C基因特异的siRNA引物,并进行同源性检索及合成。分别观察不同siRNA引物转染胃癌细胞株SGC7901后对其内源性VEGF-C mRNA和蛋白表达的影响。 结果 本实验成功将VEFG-C-siRNA导入胃癌细胞,5条siRNA都能抑制VEGF-C mRNA及蛋白的表达,但siRNA引物3、4阻断表达的效果最佳。 结论 siRNA技术可以有效地阻断胃癌细胞中内源性VEGF-C的基因及蛋白表达。  
    Abstract: Objective To explore the interfering effects of siRNA on endogenous VEGF-C genes and the protein expression in gastric cancer cells. Methods Cultured gastric cancer cell line SGC7901 cells were prepared in vitro. Five siRNA primers of VEGF-C were designed depending on the sequence of Accession No.BC035212.1 in Genbank. After homology analysis, the primers were synthesized and transfected into SGC7901 cells. The endogenous VEGF-C mRNA level and its protein expression were observed. Results VEGF-C-siRNA was inserted into the gastric cancer cell successfully. Five siRNA primers of VEGF-C could inhibit VEGF-C genes and protein expression. Conclusion siRNA could block the endogenous VEGF-C genes and the protein expression in gastric cancer cell.  
    • HTML全文
© 2013 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取🌃 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!