koko体育app

欢迎来到《koko体育app 学报（医学版）》
秦文, 邓存良, 陈文等. 灵芪蠲肝胶囊含药血清对活化大鼠肝星状细胞凋亡的影响[J]. koko体育app 学报（医学版）, 2014, 45(4): 595-600.
引用本文: 秦文, 邓存良, 陈文等. 灵芪蠲肝胶丸含药血清对纯化大鼠肝星状受损细胞凋亡的印象[J]. 湖北师范大学学报（中医药学版）, 2014, 45(4): 595-600.
QIN Wen, DENG Cun-liang, CHEN Wen. et al. Effects of LingQiJuanGan Capsule Medicated Serum on Apoptosis of Activated Rat Hepatic Stellate Cells[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(4): 595-600.
Citation: QIN Wen, DENG Cun-liang, CHEN Wen. et al. Effects of LingQiJuanGan Capsule Medicated Serum on Apoptosis of Activated Rat Hepatic Stellate Cells[J]. Journal of Sichuan University (Medical Sciences), 2014, 45(4): 595-600.

灵芪蠲肝胶囊含药血清对活化大鼠肝星状细胞凋亡的影响

Effects of LingQiJuanGan Capsule Medicated Serum on Apoptosis of Activated Rat Hepatic Stellate Cells

  • 摘要: 目的 观察灵芪蠲肝胶囊含药血清对血小板衍化生长因子 （PDGF）诱导的活化大鼠肝星状细胞 （HSC-T6）凋亡的作用，并观察其对凋亡基因Bcl-2、Bax蛋白表达的影响,探讨其可能的抗肝纤维化机制。方法 首先制备含药血清：SD大鼠随机分为3组，分别用生理盐水 （A组，10 mL/kg），复方鳖甲软肝片药液 （B组，1.5 g/kg），灵芪蠲肝胶囊药液 （C组，4.25 g/kg）灌胃7 d，每日1次。经腹主动脉采血，静置、离心、灭活、过滤，取得含药血清。以PDGF 10 ng/mL刺激活化HSC-T6。将各组含药血清按200 mL/L分别作用于HSC-T6。0、12、24、48 h后采用流式细胞技术检测各组HSC-T6凋亡情况以及Bcl-2、Bax的表达。结果 B、C组含药血清作用于HSC-T6细胞12、24、48 h，细胞凋亡率较A组升高，差异有统计学意义 （P<0.05），而B、C组间的差异无统计学意义（P>0.05）。各组组内不同时间点比较，除A组外，B、C组在不同时间点的两两比较差异均有统计学意义，细胞凋亡率随时间的延长出现明显上升 （P<0.05）。含药血清作用于HSC-T6细胞12、24、48 h，B组、C组Bcl-2的表达较A组明显下降，Bax的表达出现明显上升，差异均有统计学意义 （P<0.05），而B组与C组间差异均无统计学意义 （P>0.05）。除A组外，B组和C组在不同时间点的两两比较差异均有统计学意义 （P<0.05），Bcl-2蛋白的表达在0 h最高，随时间的延长表现为下降趋势 （P<0.05），Bax蛋白的表达随着时间的延长，整体表现为上调趋势 （P<0.05）。结论 灵芪蠲肝胶囊可以诱导活化的HSC-T6凋亡，其机制可能为影响Bcl-2/Bax表达失衡，从而发挥其抗纤维化作用。  
    Abstract: Objective To investigate the effects of LingQiJuanGan Capsule medicated serum on the apoptosis of and the expression of Bcl-2/Bax in hepatic stellate cell-T6 (HSC-T6) activated by platelet-derived growth factor (PDGF). Methods Preparation of drug-medicated serum. 30 SD rats were randomly divided into three groups. Serum was obtained from the SD rats administrated intragastricly with saline (10 mL/kg, group A), Fufang Biejia Ruangan Tablet solution (1.5 g/kg, group B) and LingQiJuanGan Capsule solution respectively (4.25 g/kg, group C). Detection of cell apoptosis and the expression of Bcl-2/Bax. HSC-T6 cells were activated by 10 ng/mL PDGF and incubated with drug-medicated serum at 200 mL/L. Cell apoptosis and the expression of Bcl-2/Bax were measured by flow cytometry at 0, 12, 24 and 48 h. Results Significantly higher apoptotic rates of HSC-T6 in group B and group C were detected at 12, 24, 48 h compared with that of group A (P<0.05 ), wheraus no significant difference was found between group B and group C (P>0.05). With increase of exposure time, the apoptotic rates of HSC-T6 in group B and group C increased (P<0.05). The levels of Bcl-2 expression in HSC-T6 in group B and C were lower than that of group A (P<0.05) at 12, 24, 48 h (No significant difference between group B and group C, P>0.05). The expression level of Bcl-2 decreased with increased exposure time (P>0.05) in both group B and group C.However, no such trend was observed in group A (P>0.05). In both group B and group C, the expression level of Bax increased in a time-dependent manner. Conclusion LingQiJuanGan Capsule can induce apoptosis of PDGF activated HSC-T6 and inhibit hepatic fibrosis in a time-dependent manner by influencing the expression of Bcl-2/Bax.  
    • HTML全文
© 2014 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取🀅 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!