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黄正接, 王锐, 罗维远等. OCT4基因在胃癌细胞系中的表达及其意义[J]. koko体育app 学报（医学版）, 2012, 43(6): 812-815.
引用本文: 黄正接, 王锐, 罗维远等. OCT4DNA在食道癌细胞系系中的描述试述含义[J]. 云南大学本科学报（医学研究版）, 2012, 43(6): 812-815.
HUANG Zheng-jie, WANG Rui, LUO Wei-yuan. et al. Expression of OCT4 in Gastric Cancer Cell Lines and Its Significance[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 812-815.
Citation: HUANG Zheng-jie, WANG Rui, LUO Wei-yuan. et al. Expression of OCT4 in Gastric Cancer Cell Lines and Its Significance[J]. Journal of Sichuan University (Medical Sciences), 2012, 43(6): 812-815.

OCT4基因在胃癌细胞系中的表达及其意义

Expression of OCT4 in Gastric Cancer Cell Lines and Its Significance

  • 摘要: 【摘要】 目的 检测八聚体结合蛋白-4（octamer-binding protein-4，OCT4）基因在不同分化程度的胃癌细胞株（MKN-28、SGC-7901、BGC-823）及正常胃黏膜细胞株GES-1中的表达情况，研究OCT4基因表达水平与胃癌细胞分化程度和侵袭力的相关性。方法 RT-PCR确定GES-1、MKN-28、SGC-7901、BGC-823中OCT4基因的表达水平，OCT4 siRNA转染BGC-823细胞，分别通过荧光定量PCR和Western blot检测干扰OCT4基因表达的效果，并利用Transwell小室法研究OCT4在BGC-823细胞侵袭力中的作用。结果 正常人胃黏膜细胞中未检测到OCT4的表达，人胃癌细胞系分化程度越低，OCT4基因表达量越高，低分化人胃癌细胞系BGC-823中OCT4基因表达最高，转染OCT4 siRNA的BGC-823细胞内的OCT4 mRNA和OCT4蛋白表达量明显下降（P＜0．05）。干扰胃癌细胞BG-823中OCT4基因的表达，穿膜细胞数量减少（P＜0．05），侵袭力下降。结论 OCT4基因表达量与胃癌细胞的分化程度有关，OCT4基因可能在胃癌细胞的分化中起重要作用，影响胃癌细胞的侵袭能力，其表达程度有望成为胃癌患者恶性度预测的参考指标之一。  
    Abstract: 【Abstract】 Objective To detect the expression of octamer-binding protein-4（OCT4）in gastric cancer cell lines with different differentiation (MKN-28, SGC-7901, BGC-823) and normal gastric mucosal cells line GES-1, and further assess the relationship between OCT4 expression and the differentiation grade of gastric carcinoma cells. Methods Expression level of OCT4 in GES-1, MKN-28, SGC-7901 and BGC-823 was detected by reverse transcription-polymerase chain reaction (RT-PCR), and OCT4 siRNA was employed to interfere OCT4 expression in BGC-823 cell lines. Detect the quantity of OCT4 mRNA and OCT4 protein by fluorescent quantitative PCR and Western blot respectively. In addition, the invasion ability was analyzed via Transwell chamber. Results The normal gastric mucosal cells did not express OCT4 and there was higher expression of OCT4 in gastric cancer cell lines with poorly differentiation (P<0.05). The expression of OCT4 in BGC-823 cells was the highest. The expression of OCT4 mRNA and OCT4 protein were decreased distinctly in BGC-823 cells after being interfered by OCT4 siRNA (P<0.05). After being interfered by OCT4, BGC-823 cells were less aggressive, and the number of penetrating cells was decreased (P<0.05). Conclusion The OCT4 expression level is associated with gastric cancer differentiation. OCT4 may play an important role in the differentiation and invasion of gastric cancer cell and it may serve as a reference index in predicting the malignant grade of gastric cancer.  
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