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余毅, 李增棋, 陈昆等. MMP-1、TIMP-1及TGF-β1在风湿性心脏病瓣膜组织中的 表达及意义[J]. koko体育app 学报（医学版）, 2017, 48(1): 52-56.
引用本文: 余毅, 李增棋, 陈昆等. MMP-1、TIMP-1及TGF-β1在痛风性左心病瓣膜组织安排中的 呈现及有何意义[J]. 甘肃读书学报（分子生物学版）, 2017, 48(1): 52-56.

MMP-1、TIMP-1及TGF-β1在风湿性心脏病瓣膜组织中的 表达及意义

  • 摘要: 目的 探讨基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶组织抑制剂-1（TIMP-1）及转化生长因子-β1（TGF-β1）在风湿性心脏病（RHD）瓣膜组织中的表达及意义。方法 采用Western blot及RT-PCR法检测RHD瓣膜组织（实验组， n=30）及无RHD瓣膜组织（对照组， n=15）中MMP-1、TIMP-1及TGF-β1蛋白与mRNA的表达，碱水解法检测胶原代谢情况，Masson法进行胶原纤维染色并检测胶原容积分数（CVF）。分析MMP-1、TIMP-1及TGF-β1与CVF的相关性。结果 MMP-1及TGF-β1蛋白和mRNA在实验组中的表达高于对照组，而TIMP-1蛋白和mRNA在实验组中的表达低于对照组，差异有统计学意义( P<0.05)。实验组胶原含量及CVF均高于对照组( P<0.05)。相关性分析显示，MMP-1与TGF-β1呈正相关，TIMP-1与TGF-β1呈负相关，MMP-1及TGF-β1与CVF呈正相关，TIMP-1与CVF呈负相关。结论 MMP-1、TGF-β1和TIMP-1参与了RHD瓣膜病变的纤维化进展。  
    Abstract: Objective To explore expressions of matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinases-1 (TIMP-1) and transforming growth factor-β1 (TGF-β1) in valve tissue of rheumatic heart disease (RHD), and to analyzed their roles in RHD. Methods The expressions of MMP-1, TIMP-1 and TGF-β1 proteins and mRNAs were tested by Western blot and RT-PCR methods in valve tissues in participants with (experimental group, n=30) and without RHD (control group, n=15). Collagen fibers were detected by Masson staining, and collagen volume fraction (CVF) was caculated. The correlations of CVF and the expressions of MMP-1, TIMP-1 and TGF-β1 were analyzed. Results The collagen fibers, CVF, and the protein and mRNA expressions of MMP-1 and TGF-β1 in experimental group were higher than those in control group, while the protein and mRNA expressions of TIMP-1 in experimental group were lower than those in control group. The expression of TIMP-1 was negatively correlated with TGF-β1 and CVF in valve tissues, while MMP-1 was positively correlated with them. The expression of TGF-β1 was positively correlated with CVF in valve tissues. Conclusion MMP-1, TIMP-1 and TGF-β1 contribute to the progression of fibrosis in RHD.  
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