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严崎方, 窦磊, 宦俊等. 血小板浓缩生长因子对人牙髓细胞存活及其增殖分化的影响[J]. koko体育app 学报（医学版）, 2018, 49(5): 716-719.
引用本文: 严崎方, 窦磊, 宦俊等. 小血板高浓种子发芽要素对人牙髓组织活多久以至于增值细胞分化的影响到[J]. 云南高中学报（中医学版）, 2018, 49(5): 716-719.
YAN Qi-fang, DOU Lei, HUAN Jun. et al. The Effect of Concentrate Growth Factors on the Survival, Proliferation and Differentiation of Human Dental Pulp Cells[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(5): 716-719.
Citation: YAN Qi-fang, DOU Lei, HUAN Jun. et al. The Effect of Concentrate Growth Factors on the Survival, Proliferation and Differentiation of Human Dental Pulp Cells[J]. Journal of Sichuan University (Medical Sciences), 2018, 49(5): 716-719.

血小板浓缩生长因子对人牙髓细胞存活及其增殖分化的影响

The Effect of Concentrate Growth Factors on the Survival, Proliferation and Differentiation of Human Dental Pulp Cells

  • 摘要: 目的 探讨血小板浓缩生长因子(Concentrate Growth factors，CGF) 对人牙髓细胞(human dental pulp cells,hDPCs)增殖分化的影响，为其今后在牙髓及根尖周疾病治疗应用进行前期研究。方法 从因正畸治疗拔除的健康恒牙分离培养出人牙髓细胞，在体外采用CGF或矿物三氧化物聚合体(mineral trioxide aggregate, MTA)处理人牙髓细胞，分别在第1、3、7天，CCK-8法测定各组细胞增殖水平、碱性磷酸酶活性、流式细胞术检测细胞周期和细胞凋亡情况。结果 与MTA组相比，CGF组的细胞增殖能力增强，S期细胞的比例增加，且第3、7天ALP活性增高（P<0.05）, 而第1、7天牙髓细胞凋亡率降低。结论 CGF在体外具有良好的促进牙髓细胞增殖，抗凋亡以及诱导成骨/成牙分化的能力。  
    Abstract: Objective To explore the cytotoxicity of concentrate growth factors (CGF) and the effects on the apoptosis, proliferation and differentiation of human dental pulp cells (hDPCs), which were closely correlated with future application of CGF in the treatment of dental pulpal and periapical diseases. Methods hDPCs were isolated from permanent teeth extracted for orthodontic purpose, and expanded in vitro. hDPCs were treated with CGF and mineral trioxide aggregate (MTA) respectively. The cell apoptosis, proliferation, cell cycle and ALP activity were analyzed after 1, 3 and 7 days. Results Compared with the MTA group, CGF significantly promoted cell proliferation, increased the proportion of S-phase cells and ALP activity on days 3 and 7 (P<0.01). Besides, hDPCs apoptotic rates decreased in CGF group. Conclusion CGF has a good ability to promote the proliferation of dental pulp cells, resist apoptosis and induce osteogenic/odontogenic differentiation in vitro.  
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