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史海涛, 师阿盟, 王燕等. CXCR7在胃癌细胞中的表达及对SGC-7901细胞迁移及侵袭的影响[J]. koko体育app 学报（医学版）, 2016, 47(5): 685-690.
引用本文: 史海涛, 师阿盟, 王燕等. CXCR7在食道癌神经元中的表达爱及对SGC-7901神经元转入及肉瘤样癌的影晌[J]. 山东社会学报（医学界版）, 2016, 47(5): 685-690.
SHI Hai-tao, SHI A-meng, WANG Yan. et alY。. Expression of CXCR7 in Gastric Cancer Cells and Its Effect on the Migration and Invasion of SGC-7901 Cells[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(5): 685-690.
Citation: SHI Hai-tao, SHI A-meng, WANG Yan. et alY。. Expression of CXCR7 in Gastric Cancer Cells and Its Effect on the Migration and Invasion of SGC-7901 Cells[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(5): 685-690.

CXCR7在胃癌细胞中的表达及对SGC-7901细胞迁移及侵袭的影响

Expression of CXCR7 in Gastric Cancer Cells and Its Effect on the Migration and Invasion of SGC-7901 Cells

  • 摘要: 目的 探讨趋化因子受体7(CXCR7)在不同分化程度的胃癌细胞系中的表达以及siRNA沉默CXCR7后对胃癌SGC-7901细胞迁移及侵袭能力的影响。方法 体外培养5种人胃癌细胞系：未分化腺癌HGC-27、低分化黏液腺癌MGC-803、低分化腺癌BGC-823、中分化腺癌SGC-7901和高分化腺癌MKN-28。采用Western blot及RT-PCR法分别检测CXCR7的蛋白及mRNA表达。应用脂质体转染siRNA的方法沉默SGC-7901细胞CXCR7的表达,并采用其配体基质细胞衍生因子-1（SDF-1）干预，实验分为4组：阴性对照siRNA(NC siRNA组), NC siRNA+SDF-1组,CXCR7 siRNA组和CXCR7 siRNA+SDF-1组。应用Transwell小室检测细胞迁移及侵袭能力。结果 CXCR7在不同人胃癌细胞系中表达程度不一，其中高分化MKN-28几乎不表达，中分化SGC-7901细胞表达程度最高。与 NC siRNA组相比，NC siRNA＋SDF-1组的迁移细胞数和侵袭细胞数增加（P CXCR7 siRNA组的迁移细胞数和侵袭细胞数减少（P CXCR7 siRNA＋SDF-1组的迁移细胞数和侵袭细胞数减少（P CXCR7 siRNA抑制。  
    Abstract: Objective To determine the expression of chemokine (C-X-C motif) receptor 7 (CXCR7) in five gastric cancer cell lines with various degrees of differentiation, and the effect of silencing CXCR7 on the migration and invasion of SGC-7901 cells. Methods The expression of CXCR7 in gastric cell lines (HGC-27, MGC-803, SGC-7901, BGC-823 and MKN-28) was detected by Western bolt and RT-PCR. The SGC-7901 cells were transfected with liposome of CXCR7 siRNA to silence CXCR7 gene, and then treated with stromal-derived factor-1 (SDF-1)——the ligand of CXCR7. Transwell assay was used for determining the migratory and invasive ability of SGC-7901 cells in the four groups: NC siRNA, NC siRNA+SDF-1, CXCR7 siRNA and CXCR7 siRNA+SDF-1. Results CXCR7 was expressed in the five gastric cancer cell lines, with the highest intensity in SGC-7901. The migrated and invasive cells increased in the NC siRNA＋SDF-1 group and reduced in the CXCR7-siRNA group compared with the NC siRNA group (P CXCR7-siRNA＋SDF-1 group had less migrated and invasive cells than the NC siRNA＋SDF-1 group (P CXCR7 siRNA .  
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