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肖永红, 梁晓飞, 王悦等. 17β-雌二醇对SiO 2刺激的小鼠肺成纤维细胞表达Caveolin-1和Ⅲ型胶原的影响[J]. koko体育app 学报（医学版）, 2015, 46(1): 62-64.
引用本文: 肖永红, 梁晓飞, 王悦等. 17β-雌二醇对SiO 2热血的小鼠肺成弹性纤维神经细胞描述Caveolin-1和Ⅲ型胶原的干扰[J]. 四川省上大学学报（中医学版）, 2015, 46(1): 62-64.
XIAO Yong-hong, LIANG Xiao-fei, WANG Yue. et al. Effects of 17β-estradiol on the Expression Caveolin-1 and Type Ⅲ Collagen in the Mouse Lung FibroblastStimulated by SiO 2[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(1): 62-64.
Citation: XIAO Yong-hong, LIANG Xiao-fei, WANG Yue. et al. Effects of 17β-estradiol on the Expression Caveolin-1 and Type Ⅲ Collagen in the Mouse Lung FibroblastStimulated by SiO 2[J]. Journal of Sichuan University (Medical Sciences), 2015, 46(1): 62-64.

17β-雌二醇对SiO 2刺激的小鼠肺成纤维细胞表达Caveolin-1和Ⅲ型胶原的影响

Effects of 17β-estradiol on the Expression Caveolin-1 and Type Ⅲ Collagen in the Mouse Lung FibroblastStimulated by SiO 2

  • 摘要: 目的 探讨不同浓度17β-雌二醇(17β-E2)对二氧化硅（SiO2）刺激的小鼠肺成纤维细胞表达Caveolin-1和Ⅲ型胶原的影响。方法 将体外培养的小鼠肺成纤维细胞分5组：空白对照组，SiO2（100 mg/L）组，SiO2（100 mg/L）+不同剂量17β-E2组（10-8、10-7、10-6 mol/L），17β-E2作用48 h后收集各组细胞，应用免疫组化法检测Caveolin-1和Ⅲ型胶原表达。结果 SiO2 处理组小鼠肺成纤维细胞与空白对照组比较Caveolin-1表达降低（P<0.05），而SiO2+ 17β-E2处理组随着17β-E2剂量增大Caveolin-1表达明显升高（P<0.05）；各处理组Ⅲ型胶原表达差异有统计学意义（P<0.05），SiO2组与空白对照组比较增高（P<0.05），而17β-E2处理组均低于SiO2组（P<0.05）；相关分析显示，17β-E2与Caveolin-1的表达呈正相关（r=0.926，P<0.05），与Ⅲ型胶原表达呈负相关（r=-0.914，P<0.05），Caveolin-1与Ⅲ型胶原表达亦呈负相关（r=-0.887，P<0.05）。结论 17β-E2可能通过上调肺成纤维细胞Caveolin-1 的表达，抑制Ⅲ型胶原表达而发挥抗纤维作用。  
    Abstract: Objective To observe the effects of different concentration of 17β-estradiol (17β-E2) on the expression of Caveolin-1 and type Ⅲ collagen in the mouse lung fibroblast stimulated by SiO2. Methods Fibroblast cells were devided into five groups: blank control group, SiO2 (100 mg/L) group and SiO2 (100 mg/L)+ different concentration of 17β-E2 (10-8，10-7，10-6 mol/L) groups. After treated with different concentration of 17β-E2, for 48 h, the cells were collected, then the expression of Caveolin-1 and of type Ⅲ collagen were examined with immunohistochemistry method. Results Compared with the blank control group, the expression of Caveolin-1 in mouse lung fibroblasts treated by SiO2 significantly decreased (P <0.05), While which in the SiO2+17β-E2 group significantly increased as 17β -E2 dose increased (P < 0.05); There were significantly different in the expression of type Ⅲ collagen among different groups (P <0.05), which in SiO2 group were evidently higher than that in the blank control group, while which in 17β-E2 groups decreased significantly, when compared with SiO2 group (P <0.05); 17β-E2 increased the expression of Caveolin-1 and decreased the expression of type Ⅲ collagen in the dose dependent manner. Correlation analysis showed that 17β-E2 was positive correlated with the expression of Caveolin-1 (r=0.926, P <0.05), and negative correlated with the express of type Ⅲ collagen (r=-0.914, P <0.05), and the expression of Caveolin-1 and the expression of type Ⅲ collagen was negatively correlation (r=-0.887, P <0.05). Conclusion 17β-E2 may inhibit the expression of type Ⅲ collagen by up-regulating the expression of Caveolin-1 in mouse lung fibroblast cell to play a role in the resistance of the lung fibrosis.  
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