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丹参酮ⅡA对雌性家兔LQT2模型心肌细胞动作电位的影响
The Effect of Tanshinone ⅡA on the Transmembrance Action Potential of Myocardial Cells in LQT2 Rabbit Models
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摘要: 目的 观察丹参酮ⅡA对雌性家兔长QT2(long QT,LQT2)模型跨左室壁不同部位心肌细胞动作电位的影响。 方法 采用全细胞膜片钳技术记录雌性家兔LQT2模型左室壁内、中、外3层心肌细胞的动作电位(action potential,AP),观察10 mg/mL丹参酮ⅡA对3层肌细胞动作电位复极达90%时程(APD90)和跨室壁复极离散度(transmural dispersion of repolarization,TDR)的影响。 结果 LQT2模型家兔左室3层心肌细胞中,中膜APD90最长,其次为内膜,最短为外膜,灌注10 mg/mL丹参酮ⅡA后能缩短LQT2模型的各层心肌细胞的APD90,同时降低TDR。 结论 10 mg/mL丹参酮ⅡA能有效缩短LQT2模型的各层心肌细胞动作电位,同时降低各层心肌细胞动作电位复极的差异性。Abstract: Objective To determine the effect of tanshinone ⅡA on the transmembrance action potential of three layers of myocardial cells in LQT2 rabbit models. Methods Whole-cell patch clamp technique was used to record the action potential duration 90 (APD90) and the transmural dispersion of repolarization(TDR) of the epicardium, mid-myocardium and endocardium in the LQT2 rabbits. Results Of the three layers of myocardial cells, the mid-myocardium had the longest APD90 and the epicardium had the shortest APD90. The APD90 of all three layers of myocardial cells were shortened with infusion of 10 mg/mL tanshinone ⅡA. Their TDR values also reduced by the infusion. Conclusion Tanshinone ⅡA shortens APD90 and reduces TDR values.
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