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张梦妍, 廖虹瑜, 范雪佳, 等. 四川呼吸道合胞病毒F蛋白基因序列和抗原表位变异分析[J]. koko体育app 学报（医学版）, 2013, 44(2): 175-178.
引用本文: 张梦妍, 廖虹瑜, 范雪佳, 等. 云南吸气道合胞病毒有哪些F核蛋白基因遗传编码序列和抗原表位遗传变异阐述[J]. 云南大学专业学报（医学研究版）, 2013, 44(2): 175-178.
ZHANG Meng-yan, LIAO Hong-yu, FAN Xue-jia, et al. Gene Sequence and Antigenic Epitope Variation within the F Protein of Respiratory Syncytial Virus in Sichuan, China[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(2): 175-178.
Citation: ZHANG Meng-yan, LIAO Hong-yu, FAN Xue-jia, et al. Gene Sequence and Antigenic Epitope Variation within the F Protein of Respiratory Syncytial Virus in Sichuan, China[J]. Journal of Sichuan University (Medical Sciences), 2013, 44(2): 175-178.

四川呼吸道合胞病毒F蛋白基因序列和抗原表位变异分析

Gene Sequence and Antigenic Epitope Variation within the F Protein of Respiratory Syncytial Virus in Sichuan, China

  • 摘要: 目的 分析呼吸道合胞病毒(RSV)四川分离株F蛋白基因序列,探讨F蛋白基因和抗原表位变异情况。 方法 RT-PCR方法扩增F蛋白近全长序列并测序,分析核苷酸和氨基酸变异位点,比较不同亚型和基因型间中和表位、细胞毒性T淋巴细胞(CTL)表位基因组成。 结果 10株RSV F蛋白的核苷酸和氨基酸P-distance分别为0.102±0.005和0.058±0.006,中和表位47F和L4在亚型间高度保守,而RS-348和7C2在亚型内保守,CTL表位HLA B*57、HLA A*01和HLA Cw*12也是亚型内高度保守,亚型间存在个别氨基酸变异。 结论 RSV四川分离株F蛋白基因保守度较高,抗原表位在亚型内保守,已在A亚型上识别的CTL表位可能在B亚型内不能识别。  
    Abstract: Objective To investigate the variation of cytotoxic T-lymphocyte (CTL) and neutralizing epitopes in F protein of respiratory syncytial virus (RSV) isolated in Sichuan. Methods Nearly full-length of F protein gene of 10 strains of RSV isolated in Sichuan was amplified by RT-PCR and sequenced. The genetic variations, especially the CTL and neutralizing antibody epitopes within different subtypes and genotypes were analyzed and compared. Results The F protein of RSV is highly conserved within the two subtypes, with the P-distances of nucleotide and amino acids were 0.102±0.005 and 0.058±0.006, respectively. Neutralizing epitopes 47F and L4 were conserved between the subtypes, but RS-348 and 7C2 were only conserved within the subtypes. CTL epitopes HLA B*57,HLA A *01 and HLA Cw*12 were conserved only within subtype A. There were specific different sites between the subtypes. Conclusion The sequences of F protein from Sichuan RSV isolates were highly conserved, so as the epitopes on F-protein within subtypes, the identified CLT epitopes in subtype A may not be recognized in subtype B virus.  
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