koko体育app

欢迎来到《koko体育app 学报（医学版）》
杨蕗璐, 马琳, 王红等. 大鼠70%肝切除后门静脉血流动力学变化及其与肝再生关系初探[J]. koko体育app 学报（医学版）, 2016, 47(3): 326-330.
引用本文: 杨蕗璐, 马琳, 王红等. 大鼠70%肝做手术车辆后门血管血供动力机学变举例与肝粉碎的关系浅析[J]. 北京大学本科学报（医学研究版）, 2016, 47(3): 326-330.
YANG Lu-lu, MA Lin, WANG Hong.et al. A Preliminary Study on Portal Vein Hemodynamic Changes and Liver Regeneration in Rats after 70% Partial Hepatectomy[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(3): 326-330.
Citation: YANG Lu-lu, MA Lin, WANG Hong.et al. A Preliminary Study on Portal Vein Hemodynamic Changes and Liver Regeneration in Rats after 70% Partial Hepatectomy[J]. Journal of Sichuan University (Medical Sciences), 2016, 47(3): 326-330.

大鼠70%肝切除后门静脉血流动力学变化及其与肝再生关系初探

A Preliminary Study on Portal Vein Hemodynamic Changes and Liver Regeneration in Rats after 70% Partial Hepatectomy

  • 摘要: 目的 初步探究大鼠70%肝切除（PH）后门静脉血流动力学变化及其与肝再生的关系。方法 将大鼠随机分为假手术组和70%PH组，于术前及术后第1、3、7、14天采用5.0~12.0 MHz高频超声探头测量门静脉主干管径（PVD）及最大血流速度(PVV)；并观察肝组织形态学变化及细胞增殖核抗原（PCNA）表达, 计算肝脏再生率（LRR）。结果 大鼠70%PH术后第1天，PCNA开始升高，肝细胞以空泡样变为主，肝窦受压变窄，PVD增粗, PVV减慢；第3天，肝细胞达核分裂高峰期，PCNA达峰值，肝窦结构紊乱，PVD达峰值而PVV降至最低；术后第14天，肝细胞形态和肝小叶结构逐渐恢复，PCNA恢复至假手术组水平，LRR增至90%以上，同时，PVD、PVV均恢复至假手术组水平（P>0.05）。结论 大鼠PH后血流动力学参数PVD、PVV与肝细胞的病理改变、核分裂状态、再生肝脏体积等因素有关，为超声应用于临床监测肝细胞再生的深入研究提供了基础依据。  
    Abstract: Objective To explore portal vein hemodynamic changes and liver regeneration in rats after 70% partial hepatectomy (PH). Methods Rats were randomly subjected into sham operation group and 70%PH group. A 5-12 MHz high-frequency linear transducer was applied to measure the portal vein diameter (PVD) and the maximum portal vein velocity (PVV) before and after operation on day 1, 3, 7, 14. On the corresponding time, liver tissue morphological changes were observed, and the expression level of PCNA was measured, and the liver regeneration rate (LRR) was calculated. Results On day 1 after PH, PCNA began to increase, and hepatocyte vacuolated obviously which squeezed and narrowed the hepatic sinusoid. Meanwhile, PVD started to dilate and PVV decelerated. On day 3 after operation, PCNA peaked and active mitosis resulted in the disorders of hepatic structure. PVD peaked and PVV decelerated to the lowest. Until the 14 d after operation, the cell morphology, lobular structure and PCNA recovered gradually accompany by LRR above 90%. PVD and PVV also almost recovered to levels closed to sham group (P<0.05). Conclusion The hemodynamic parameters PVD, PVV correlated with hepatic pathology changes, mitosis status and regenerated liver volume after PH, which state the potential possibility for ultrasound image study in liver regeneration surveillance.  
    • HTML全文
© 2016 《koko体育app 学报（医学版）》编辑部　版权所有 cc

开放获取🎃 本文遵循知识共享署名—非商业性使用4.0国际许可协议（CC BY-NC 4.0），允许第三方对本刊发表的论文自由共享（即在任何媒介以任何形式复制、发行原文）、演绎（即修改、转换或以原文为基础进行创作），必须给出适当的署名，提供指向本文许可协议的链接，同时标明是否对原文作了修改；不得将本文用于商业目的。CC BY-NC 4.0许可协议详情请访问

/

返回文章
返回
var _hmt = _hmt || []; (function() { var hm = document.createElement("script"); hm.src = "https://hm.baidu.com/hm.js?90c4d9819bca8c9bf01e7898dd269864"; var s = document.getElementsByTagName("script")[0]; s.parentNode.insertBefore(hm, s); })(); !function(p){"use strict";!function(t){var s=window,e=document,i=p,c="".concat("https:"===e.location.protocol?"https://":"http://","sdk.51.la/js-sdk-pro.min.js"),n=e.createElement("script"),r=e.getElementsByTagName("script")[0];n.type="text/javascript",n.setAttribute("charset","UTF-8"),n.async=!0,n.src=c,n.id="LA_COLLECT",i.d=n;var o=function(){s.LA.ids.push(i)};s.LA?s.LA.ids&&o():(s.LA=p,s.LA.ids=[],o()),r.parentNode.insertBefore(n,r)}()}({id:"K9y7iMpaU8NS42Fm",ck:"K9y7iMpaU8NS42Fm"}); koko体育-koko体育app koko体育-koko体育网页版koko体育app koko体育-全站app下载(官网) m6米乐app|下载 m6米乐app|主頁欢迎您!!